Collagen type II is downregulated in the degenerative nucleus pulposus and contributes to the degeneration and apoptosis of human nucleus pulposus cells

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Collagen type II is downregulated in the degenerative nucleus pulposus and contributes to the degeneration and apoptosis of human nucleus pulposus cells

Degenerative disc disease (DDD) is a common degenerative condition initiated mainly within the nucleus pulposus (NP). To date, the etiopathogenesis of DDD remains unclear, and because no effective therapeutic strategies are available to target its pathological processes, DDD is still treated with symptomatic interventions that are far from adequate. Collagen type II is one of the major matrix c...

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MicroRNA-494 promotes apoptosis and extracellular matrix degradation in degenerative human nucleus pulposus cells

PURPOSE This study investigated the expression and function of the microRNA-494 in intervertebral disc degeneration (IDD). RESULTS MicroRNA-494 expression was upregulated during IDD progression; its overexpression increased the expression of ECM catabolic factors such as matrix metalloproteinase and A disintegrin and metalloproteinase with thrombospondin motif in NP cells while decreasing tha...

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IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells

IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cy...

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SIRT1 protects against apoptosis by promoting autophagy in degenerative human disc nucleus pulposus cells

SIRT1 could protect degenerative human NP cells against apoptosis, and there were extensive and intimate connection between apoptosis and autophagy. Up to now, the role of autophagy in the process of human IVD degeneration is unclear. We sought to explore the relationship between autophagy and human IVD degeneration and to understand whether autophagy is involved in the protective effect of SIR...

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The Comparative Cytomorphology of Nucleus Pulposus Cells

INTRODUCTION The cells of the embryonic notochord play an important role in formation of the spinal column, including the intervertebral discs (IVDs) (1). These cells become trapped inside the annulus fibrosus and reside within the nucleus pulposus at birth, but are generally lost during aging and replaced by small chondrocyte-like cells. The age at which the notochordal cells are lost varies s...

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ژورنال

عنوان ژورنال: Molecular Medicine Reports

سال: 2017

ISSN: 1791-2997,1791-3004

DOI: 10.3892/mmr.2017.7178